Fluorescence Microscopy Digital Image Gallery
Indian Muntjac Deer Skin Fibroblast Cells
In order to label the intermediate filaments in the log phase adherent Indian Muntjac fibroblast cell culture presented above, the fixed and permeabilized cells were blocked and treated with mouse anti-vimentin (porcine eye lens) primary antibodies followed by goat anti-mouse secondary antibodies (IgG) conjugated to Texas Red-X. Filamentous actin was visualized with phalloidin conjugated to Alexa Fluor 350, while the nuclei were counterstained with SYTOX Green. Images were recorded in grayscale with a Hamamatsu ORCA-AG camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Semrock. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.