Fluorescence Microscopy Digital Image Gallery
Embryonic Rat Thoracic Aorta Cells (A7r5 Line)
Embryonic Rat Thoracic Aorta Cells (A7r5 Line)

An adherent culture of rat thoracic aorta cells (A7r5 line) was transferred to an incubation dish and treated with MitoTracker Red CMXRos and Hoechst 33258 for live-cell imaging. This combination of fluorophores highlights the proximity of nuclei and mitochondria in living cells. Images were recorded in grayscale with a Hamamatsu ORCA-AG camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Semrock. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.


View a smaller image of the A7r5 cells.