Fluorescence Microscopy Digital Image Gallery
Bovine Pulmonary Artery Endothelial Cells (BPAE Line)
A culture of BPAE cells was fixed, permeabilized, and blocked with 10-percent normal goat serum in phosphate-buffered saline prior to immunofluorescent labeling with rabbit primary antibodies to giantin, a protein resident in the Golgi complex of mammalian cells. The culture was subsequently stained with a mixture of goat anti-rabbit secondary antibody fragments (heavy and light chain) conjugated to Alexa Fluor 488. In addition, the cells were labeled with Alexa Fluor 568 conjugated to phalloidin (targeting the filamentous actin network), and for DNA with Hoechst 33342. Images were recorded in grayscale with a Hamamatsu ORCA-AG camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Semrock. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.