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Electron Multiplying Charge-Coupled Devices (EMCCDs)
By incorporating on-chip multiplication gain, the electron multiplying CCD achieves, in an all solid-state sensor, the single-photon detection sensitivity typical of intensified or electron-bombarded CCDs at much lower cost and without compromising the quantum efficiency and resolution characteristics of the conventional CCD structure.
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Transformed African Green Monkey Kidney Fibroblast Cells (COS-7 Line)
The culture of transformed African green monkey kidney cells featured in the digital image above was labeled with MitoTracker Red CMXRos (pseudocolored yellow), which selectively binds to targets in the mitochondria. The cells were also stained with Alexa Fluor 568 conjugated to phalloidin and the DNA-binding reagent SYTOX Green, which target the cytoskeletal filamentous actin network and nuclear DNA, respectively. Images were recorded in grayscale with a Hamamatsu ORCA-AG camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Semrock. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.