Fluorescence Microscopy Digital Image Gallery
Tahr Ovary Epithelial Cells (HJ1.Ov)
Employing a combination of ultraviolet, blue, and green excitation Alexa Fluor (350, 488, and 568, respectively) dyes, the monolayer culture of HJ1.Ov cells illustrated above was triple-labeled using double immunofluorescence and a phallotoxin. Nuclei were visualized with mouse anti-NPCP (nuclear pore complex protein) primary antibodies, while the Golgi complex was stained with rabbit anti-giantin antibodies. Secondary antibodies were goat anti-mouse and anti-rabbit conjugated to Alexa Fluor 488 and 568. The filamentous actin network was counterstained with Alexa Fluor 350 conjugated to phalloidin. Images were recorded in grayscale with a Hamamatsu ORCA-AG camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Omega Optical. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.