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Electron Multiplying Charge-Coupled Devices (EMCCDs)
By incorporating on-chip multiplication gain, the electron multiplying CCD achieves, in an all solid-state sensor, the single-photon detection sensitivity typical of intensified or electron-bombarded CCDs at much lower cost and without compromising the quantum efficiency and resolution characteristics of the conventional CCD structure.
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Indian Muntjac Deer Skin Fibroblast Cells
The Indian Muntjac cells presented above were resident in an adherent culture stained for DNA with the bis-benzimidazole dye Hoechst 33258 (blue fluorescence). In addition, the culture was immunofluorescently labeled with Alexa Fluor 488 (green fluorescence) and Alexa Fluor 568 (pseudocolored red) conjugated to goat secondary antibodies that target mouse anti-fibrillarin (nucleoli) and rabbit anti-giantin (targeting the Golgi complex) primary antibodies, respectively. Images were recorded in grayscale with a Hamamatsu ORCA-AG camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Semrock. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.