Because actin microfilaments are a primary component of the cytoskeletal network and are significantly involved in a number of cellular activities, it is important for a wide range of scientific studies to be able to adequately visualize filamentous actin in living cells. To meet this need, several markers have been developed for fluorescently targeting actin. Two such markers are alpha-actinin and Lifeact, which are often used in chimeras for labeling the actin cytoskeleton in live cells. In the digital video sequences featured in this section, cytoskeletal actin is targeted with both mEmerald fused to alpha-actinin and a mCherry-Lifeact fusion. Overlap between the fluorescent markers is clearly visible.