Zyxin is a zinc-binding phosphoprotein that in humans is encoded by the ZYX gene. In live cells, zyxin concentrates at focal adhesions and along the intracellular actin cytoskeletal network. Focal adhesions are located at points of contact between cells and the extracellular matrix. The structures are rich in actin and are the collecting sites of protein complexes necessary for signal transduction. To produce the digital video sequences presented in this section, fox lung cells were labeled with mCherry fused to Zyxin and mEmerald fused to Lifeact.