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EMCCDs Article Electron Multiplying Charge-Coupled Devices (EMCCDs)

By incorporating on-chip multiplication gain, the electron multiplying CCD achieves, in an all solid-state sensor, the single-photon detection sensitivity typical of intensified or electron-bombarded CCDs at much lower cost and without compromising the quantum efficiency and resolution characteristics of the conventional CCD structure.

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Mitochondria and Microtubule EB3


Objective: UPlanSApo 100x oil/1.40 Exposure: 250 ms
Microscope: Olympus DSU/IX81 Gain: 3
Camera: Hamamatsu ImagEM Interval: 500 ms

Microtubules are straight, hollow cylinders found throughout the cytoplasm of all eukaryotic cells (prokaryotes don't have them) and carry out a variety of functions, ranging from transport to structural support. Microtubules, which are about 25 nanometers in diameter, form part of the cytoskeleton that gives structure and shape to a cell, and also serve as conveyor belts moving other organelles throughout the cytoplasm. In addition, microtubules are the major components of cilia and flagella, and participate in the formation of spindle fibers during cell division (mitosis). The length of microtubules in the cell varies between 200 nanometers and 25 micrometers, depending upon the task of a particular microtubule and the state of the cell's life cycle. Microtubules are believed to act as a transport mechanism for mitochondria. The digital video in this section illustrates the interplay between mitochondria (labeled with DsRed fluorescent protein) and the microtubules by examining the tracking of microtubule +TIPs (plus end tracking proteins) labeled with EB3 fused to mEGFP.

BACK TO FoLu CELLS WITH DsRED-MITOCHONDRIA AND mEGFP-EB3