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Electron Multiplying Charge-Coupled Devices (EMCCDs)
By incorporating on-chip multiplication gain, the electron multiplying CCD achieves, in an all solid-state sensor, the single-photon detection sensitivity typical of intensified or electron-bombarded CCDs at much lower cost and without compromising the quantum efficiency and resolution characteristics of the conventional CCD structure.
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Mitochondria and Endoplasmic Reticulum in Epithelial Cells
The digital videos in this section examine owl monkey kidney epithelial cells (OMK line) that were transfected with a mixture of mEGFP fused to a mitochondrial targeting signal (green fluorescence) and DsRed fluorescent protein fused to an endoplasmic reticulum targeting signal (red fluorescence). The dual labeling enables the observation of the complex interplay between mitochondria and the endoplasmic reticulum. Mitochondria are similar to plant chloroplasts in that both organelles are able to produce energy and metabolites that are required by the host cell. The endoplasmic reticulum is centrally involved in cellular protein processing.
Video 1 - Run Time: 38 Seconds - The increased concentration and activity of mitochondria near the endoplasmic reticulum is clearly visible in a single owl monkey kidney cell. Choose a playback version: Streaming Video (7.1 MB), Progressive Download (7.1 MB), and MPEG Download (17 MB)
Video 2 - Run Time: 25 Seconds - The proximity of cytoplasmic elements present in an OMK cell provide an excellent window to the activity of mitochondria. Choose a playback version: Streaming Video (4.7 MB), Progressive Download (4.7 MB), and MPEG Download (17 MB)