By incorporating on-chip multiplication gain, the electron multiplying CCD achieves, in an all solid-state sensor, the single-photon detection sensitivity typical of intensified or electron-bombarded CCDs at much lower cost and without compromising the quantum efficiency and resolution characteristics of the conventional CCD structure.
Explore image capture with an EMCCD camera using this interactive tutorial. The live image is displayed in the upper right-hand corner, while the captured image is presented in the lower right, just above the sliders. The EMCCD acquires photons in the Image Area and transfers them to the Storage Area prior to readout. All of the specimens are labeled with a green or yellow fluorescent protein (green signal). Noise is represented as red (dark current) or blue (multiplier noise) photoelectrons. The tutorial is activated upon clicking the Capture button and can be halted at any instant using the Pause button. The Gain Level slider controls the amount of multiplication in the Gain Register. New specimens can be selected using the pull-down menu.