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Electron Multiplying Charge-Coupled Devices (EMCCDs)
By incorporating on-chip multiplication gain, the electron multiplying CCD achieves, in an all solid-state sensor, the single-photon detection sensitivity typical of intensified or electron-bombarded CCDs at much lower cost and without compromising the quantum efficiency and resolution characteristics of the conventional CCD structure.
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Tahr Ovary Epithelial Cells (HJ1.Ov)
A log-phase culture of HJ1.Ov cells was fixed, permeabilized, blocked with 10-percent normal goat serum and treated with mouse anti-vimentin primary antibodies followed by goat anti-mouse secondary Fab fragments conjugated to Alexa Fluor 568 (pseudocolored red). The cells were counterstained with phalloidin conjugated to Alexa Fluor 633 (yellow) and SYTOX Green (green). Images were recorded in grayscale with a Hamamatsu ORCA-AG camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Omega Optical. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.