Objective: UPlanSApo 100x oil/1.40	Exposure: 400 ms
Microscope: Olympus DSU/IX81	Gain: 3
Camera: Hamamatsu ImagEM	Interval: 500 ms

Microtubules are biopolymers that are composed of subunits made from an abundant globular cytoplasmic protein known as tubulin. Each subunit of the microtubule is made of two slightly different but closely related simpler units called alpha-tubulin and beta-tubulin that are bound very tightly together to form heterodimers. In a microtubule, the subunits are organized in such a way that they all point the same direction to form 13 parallel protofilaments. This organization gives the structure polarity, with only the alpha-tubulin proteins exposed at one end and only beta-tubulin proteins at the other. In the digital video presented above, Gray fox lung fibroblast cells (FoLu line) were transiently transfected with a fusion construct of mEGFP and human alpha-tubulin to highlight microtubules (green fluorescence) in order to visualize dynamic processes.