Fluorescence Microscopy Digital Image Gallery
Madin-Darby Canine Kidney Epithelial Cells (MDCK Line)
In the digital image presented above, epithelial cell tight junctions and nuclear pore complex proteins were simultaneously imaged in MDCK cells with a cocktail of mouse anti-NPCP and rabbit anti-ZO-3 primary antibodies, followed by goat anti-mouse and anti-rabbit secondary antibodies conjugated to Alexa Fluor 488 and Alexa Fluor 568, respectively. Images were recorded in grayscale with a Hamamatsu ORCA-AG camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Semrock. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.