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EMCCDs Article Electron Multiplying Charge-Coupled Devices (EMCCDs)

By incorporating on-chip multiplication gain, the electron multiplying CCD achieves, in an all solid-state sensor, the single-photon detection sensitivity typical of intensified or electron-bombarded CCDs at much lower cost and without compromising the quantum efficiency and resolution characteristics of the conventional CCD structure.

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Fluorescence Microscopy Digital Image Gallery
Embryonic Rat Thoracic Aorta Cells (A7r5 Line)
Embryonic Rat Thoracic Aorta Cells (A7r5 Line)

The peroxisome organelles present in the rat thoracic aorta fibroblast cell culture illustrated above were immunofluorescently labeled with Rhodamine Red-X conjugated to antibodies directed against peroxisomal membrane protein 70 (PMP 70). Alexa Fluor 488 conjugated to phalloidin, MitoTracker Red CMXRos and Hoechst 33258 were simultaneously used to counterstain the culture, targeting F-actin, mitochondria and DNA, respectively. Images were recorded in grayscale with a Hamamatsu ORCA-AG camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Semrock. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

View a larger image of the A7r5 cells.