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EMCCDs Article Electron Multiplying Charge-Coupled Devices (EMCCDs)

By incorporating on-chip multiplication gain, the electron multiplying CCD achieves, in an all solid-state sensor, the single-photon detection sensitivity typical of intensified or electron-bombarded CCDs at much lower cost and without compromising the quantum efficiency and resolution characteristics of the conventional CCD structure.

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ORCA-Flash 2.8 Digital Video Gallery

Pig kidney cells with mEmerald-EB3 and mCherry-H2B

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The digital video sequences presented in this section feature epithelial cells undergoing mitosis as visualized through the highly dynamic interactions between fluorescently labeled red histones (mCherry) and green EB3 (mEmerald). The fluorescent protein known as mEmerald is a high-performance version of EGFP that is significantly brighter and also matures faster. When coupled to the microtubule end-binding protein EB3, traces of the fluorescently labeled positive microtubule ends can be observed traversing the cytoplasm and forming the spindle during mitosis. Compare these digital videos with those of EGFP-tubulin presented in another section.