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EMCCDs Article Electron Multiplying Charge-Coupled Devices (EMCCDs)

By incorporating on-chip multiplication gain, the electron multiplying CCD achieves, in an all solid-state sensor, the single-photon detection sensitivity typical of intensified or electron-bombarded CCDs at much lower cost and without compromising the quantum efficiency and resolution characteristics of the conventional CCD structure.

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Fluorescence Microscopy Digital Image Gallery
Bovine Pulmonary Artery Endothelial Cells (BPAE Line)
Bovine Pulmonary Artery Endothelial Cells (BPAE Line)

Endothelial cells resident in a monolayer culture were immunofluorescently labeled with Alexa Fluor 488 conjugated to goat secondary antibodies that target mouse anti-NPCP (nuclear pore complex protein; green fluorescence). The cells were also counterstained with MitoTracker Red CMXRos (mitochondria; red fluorescence) and Alexa Fluor 350 conjugated to phalloidin (filamentous actin; blue). Images were recorded in grayscale with a Hamamatsu ORCA-AG camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Omega Filters. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

View a larger image of the BPAE cells.