The localization of mEmerald fused to EB3 is demonstrated in the digital video sequences presented in this section. EB3 is a member of the family of end-binding proteins (known as EBs) that specifically associate with the ends of growing microtubules in a variety of cell types. Researchers generally believe that these proteins regulate microtubule dynamics and the binding of microtubules to organelles, membrane components, and other protein complexes. In the featured digital video sequences, the relationship between microtubules and focal adhesions, which were visualized with mApple fused to Zyxin, is explored.