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EMCCDs Article Electron Multiplying Charge-Coupled Devices (EMCCDs)

By incorporating on-chip multiplication gain, the electron multiplying CCD achieves, in an all solid-state sensor, the single-photon detection sensitivity typical of intensified or electron-bombarded CCDs at much lower cost and without compromising the quantum efficiency and resolution characteristics of the conventional CCD structure.

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Pig Kidney (LLC-PK1) Cells with mEmerald-ER and mApple-H2B

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The region of the ER studded with ribosomes is termed the rough endoplasmic reticulum. The ribosomes are responsible for assembling amino acids into protein units to be sent into the rough ER for additional processing. When the proteins are ready they are either sent to regions of the cell where they are required or transported to the Golgi apparatus for additional modification. The majority of the proteins exiting the endoplasmic reticulum are exported in vesicles that bud from the smooth portion of the ER, which is typically significantly less extensive than the rough ER. The endoplasmic reticulum was visualized in the pig kidney cells appearing in the digital video sequences in this section with mEmerald fused to an ER targeting signal. The nucleus was simultaneously highlighted with mApple fused to H2B histones.

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