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EMCCDs Article Electron Multiplying Charge-Coupled Devices (EMCCDs)

By incorporating on-chip multiplication gain, the electron multiplying CCD achieves, in an all solid-state sensor, the single-photon detection sensitivity typical of intensified or electron-bombarded CCDs at much lower cost and without compromising the quantum efficiency and resolution characteristics of the conventional CCD structure.

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ORCA-R2 Digital Video Gallery

Human Osteosarcoma Epithelial (U2OS) Cells with mApple-H2B and mEmerald-Lifeact

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Actin microfilaments are a key component of the cytoskeletal network and play an important role in a number of cellular activities. Thus, the ability to visualize F-actin in living cells is crucial to the success of a wide variety of scientific investigations and experiments. Accordingly, a number of markers have been developed for live cell targeting of the actin cytoskeleton. One of the most recent additions to the researcher's arsenal of tools for this purpose is a peptide 17 amino acids long derived from yeast Abp140p called Lifeact. Studies indicate that the unique properties of Lifeact, including its small size and low binding affinity for F-actin, enable chimeric markers constructed with it to be used with live cells without impacting actin-dependent processes. In the digital video sequences presented in this section, F-actin is visualized with a mEmerald-Lifeact fusion and H2B is fluorescently labeled with mApple.