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EMCCDs Article Electron Multiplying Charge-Coupled Devices (EMCCDs)

By incorporating on-chip multiplication gain, the electron multiplying CCD achieves, in an all solid-state sensor, the single-photon detection sensitivity typical of intensified or electron-bombarded CCDs at much lower cost and without compromising the quantum efficiency and resolution characteristics of the conventional CCD structure.

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Gray Fox Lung Fibroblast Cells with mEGFP-Labeled Mitochondria

The number of mitochondria present in a cell depends upon the metabolic requirements of that cell, and may range from a single large mitochondrion to thousands of the organelles. In the digital videos presented in this section, normal Gray fox lung fibroblast cells (FoLu line) are expressing monomeric green fluorescent protein (mEGFP) fused to the mitochondrial targeting signal from subunit VIII of human cytochrome C oxidase.

Video 1 - Run Time: 41 Seconds - Numerous mEGFP-labeled mitochondria are visualized in a single fox lung cell. Choose a playback version: Streaming Video (7.7 MB), Progressive Download (7.7 MB), and MPEG Download (71 MB)

Video 2 - Run Time: 41 Seconds - A multitude of mEGFP-labeled mitochondria are active around the nucleus of a single fox lung cell. Choose a playback version: Streaming Video (7.7 MB), Progressive Download (7.7 MB), and MPEG Download (71 MB)

Video 3 - Run Time: 21 Seconds - A single fox lung cell expresses mEGFP-labeled mitochondria. Choose a playback version: Streaming Video (1.8 MB), Progressive Download (1.8 MB), and MPEG Download (36 MB)

Video 4 - Run Time: 21 Seconds - A sparse quantity of mEGFP-labeled mitochondria can be visualized in a single fox lung cell. Choose a playback version: Streaming Video (2.1 MB), Progressive Download (2.1 MB), and MPEG Download (36 MB)

Video 5 - Run Time: 15 Seconds - Isolated mitochondria fuse and fragment in the cytoplasm of a single fibroblast cell. Choose a playback version: Streaming Video (2.1 MB), Progressive Download (2.1 MB), and MPEG Download (25 MB)

Video 6 - Run Time: 41 Seconds - Numerous mEGFP-labeled mitochondria surround the nucleus of a single fox lung cell. Choose a playback version: Streaming Video (7.7 MB), Progressive Download (7.7 MB), and MPEG Download (71 MB)

Video 7 - Run Time: 21 Seconds - Numerous mEGFP-labeled mitochondria form the shape of a stick-figure bird in a single fox lung cell. Choose a playback version: Streaming Video (2.1 MB), Progressive Download (2.1 MB), and MPEG Download (36 MB)

Video 8 - Run Time: 21 Seconds - Densely-packed mEGFP-labeled mitochondria aggregate next to the nucleus of a single fox lung cell. Choose a playback version: Streaming Video (3.7 MB), Progressive Download (3.7 MB), and MPEG Download (36 MB)

Video 9 - Run Time: 16 Seconds - Numerous mEGFP-labeled mitochondria can be observed in a fox lung cell. Choose a playback version: Streaming Video (2.7 MB), Progressive Download (2.7 MB), and MPEG Download (27 MB)

Video 10 - Run Time: 21 Seconds - Mitochondrial transport through the cytoplasm is readily visualized in a single fox lung fibroblast cell. Choose a playback version: Streaming Video (3.7 MB), Progressive Download (3.7 MB), and MPEG Download (36 MB)

Video 11 - Run Time: 11 Seconds - Visualized at low magnification, the mitochondria in a fibroblast cell are transported through the cytoplasm. Choose a playback version: Streaming Video (1.7 MB), Progressive Download (1.7 MB), and MPEG Download (19 MB)

Video 12 - Run Time: 21 Seconds - Mitochondria cluster around the periphery of the nucleus in a fibroblast cell and slowly migrate into the cytoplasm. Choose a playback version: Streaming Video (3.7 MB), Progressive Download (3.7 MB), and MPEG Download (36 MB)

Video 13 - Run Time: 21 Seconds - Numerous smaller mitochondria can be observed moving rapidly through the cytoplasm in this time-lapse imaging sequence. Choose a playback version: Streaming Video (3.7 MB), Progressive Download (3.7 MB), and MPEG Download (36 MB)

Video 14 - Run Time: 6 Seconds - Inter-mitochondrial interactions can be observed in this high-magnification video clip. Choose a playback version: Streaming Video (1 MB), Progressive Download (1 MB), and MPEG Download (10 MB)

Video 15 - Run Time: 9 Seconds - A large number of mitochondria are oriented in a similar direction in a mobile fox lung fibroblast cell. Choose a playback version: Streaming Video (1.5 MB), Progressive Download (1.5 MB), and MPEG Download (15 MB)

Video 16 - Run Time: 21 Seconds - Visualized at low magnification, the mitochondria in a fibroblast cell are transported through the cytoplasm. Choose a playback version: Streaming Video (3.7 MB), Progressive Download (3.7 MB), and MPEG Download (36 MB)

Video 17 - Run Time: 21 Seconds - Numerous mEGFP-labeled mitochondria can be observed in a fox lung cell. Choose a playback version: Streaming Video (3.7 MB), Progressive Download (3.7 MB), and MPEG Download (36 MB)

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