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EMCCDs Article Electron Multiplying Charge-Coupled Devices (EMCCDs)

By incorporating on-chip multiplication gain, the electron multiplying CCD achieves, in an all solid-state sensor, the single-photon detection sensitivity typical of intensified or electron-bombarded CCDs at much lower cost and without compromising the quantum efficiency and resolution characteristics of the conventional CCD structure.

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Microtubule EB3 in Fibroblast Cells

Objective: UPlanSApo 100x oil/1.40 Exposure: 100 ms
Microscope: Olympus DSU/IX81 Gain: 3
Camera: Hamamatsu ImagEM Interval: 2 s

Microtubules are biopolymers that are composed of subunits made from an abundant globular cytoplasmic protein known as tubulin. Each subunit of the microtubule is made of two slightly different but closely related simpler units called alpha-tubulin and beta-tubulin that are bound very tightly together to form heterodimers. In a microtubule, the subunits are organized in such a way that they all point the same direction to form 13 parallel protofilaments. This organization gives the structure polarity, with only the alpha-tubulin proteins exposed at one end and only beta-tubulin proteins at the other. The digital video in this section illustrates the tracking of microtubule +TIPs (plus end tracking proteins) in Gray fox lung fibroblast cells labeled with a chimera of microtubule end-binding protein EB3 fused to monomeric Kusabira Orange fluorescent protein, which is pseudocolored red for optimum visualization.