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Electron Multiplying Charge-Coupled Devices (EMCCDs)
By incorporating on-chip multiplication gain, the electron multiplying CCD achieves, in an all solid-state sensor, the single-photon detection sensitivity typical of intensified or electron-bombarded CCDs at much lower cost and without compromising the quantum efficiency and resolution characteristics of the conventional CCD structure.
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Epithelial Cell Mitosis
 Objective: UPlanSApo 100x oil/1.40 |
Exposure: 100 ms |
| Microscope: Olympus DSU/IX81 |
Gain: 3 |
| Camera: Hamamatsu ImagEM |
Interval: 7 min |
Mitosis, a phenomenon observed in all higher eukaryotes, is the mechanism that allows the nuclei of cells to split and provide each daughter cell with a complete set of chromosomes during cellular division. This, coupled with cytokinesis (division of the cytoplasm), occurs in all multicellular plants and animals to permit growth of the organism. Digital imaging with fluorescence microscopy is becoming a powerful tool to assist scientists in understanding the complex process of mitosis on both a structural and functional level. In the digital video presented in this section, pig kidney epithelial cells (LLC-PK1 line) are expressing mCherry fluorescent protein fused to human histone H2B (red fluorescence) and mEGFP fused to the microtubule end-binding protein, EB3 (green fluorescence).