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EMCCDs Article Electron Multiplying Charge-Coupled Devices (EMCCDs)

By incorporating on-chip multiplication gain, the electron multiplying CCD achieves, in an all solid-state sensor, the single-photon detection sensitivity typical of intensified or electron-bombarded CCDs at much lower cost and without compromising the quantum efficiency and resolution characteristics of the conventional CCD structure.

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Imaging the Golgi Complex

Objective: UPlanSApo 100x oil/1.40 Exposure: 200 ms
Microscope: Olympus DSU/IX81 Gain: 3
Camera: Hamamatsu ImagEM Interval: 5 s

The Golgi apparatus (GA), also called Golgi body or Golgi complex and found universally in both plant and animal cells, is typically comprised of a series of five to eight cup-shaped, membrane-covered sacs called cisternae that look something like a stack of deflated balloons. In some unicellular flagellates, however, as many as 60 cisternae may combine to make up the Golgi apparatus. Similarly, the number of Golgi bodies in a cell varies according to its function. Animal cells generally contain between ten and twenty Golgi stacks per cell, which are linked into a single complex by tubular connections between cisternae. This complex is usually located close to the cell nucleus. In the digital video presented in this section, opossum kidney epithelial cells (OK line) are visualized expressing a fusion of mEGFP with a targeting signal to localize the chimera to the Golgi apparatus.