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EMCCDs Article Electron Multiplying Charge-Coupled Devices (EMCCDs)

By incorporating on-chip multiplication gain, the electron multiplying CCD achieves, in an all solid-state sensor, the single-photon detection sensitivity typical of intensified or electron-bombarded CCDs at much lower cost and without compromising the quantum efficiency and resolution characteristics of the conventional CCD structure.

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Mitochondria and Endoplasmic Reticulum in Epithelial Cells

Objective: UPlanSApo 100x oil/1.40 Exposure: 300 ms
Microscope: Olympus DSU/IX81 Gain: 3
Camera: Hamamatsu ImagEM Interval: 1 s

Mitochondria are similar to plant chloroplasts in that both organelles are able to produce energy and metabolites that are required by the host cell. Mitochondria are the sites of respiration, and generate chemical energy in the form of ATP by metabolizing sugars, fats, and other chemical fuels with the assistance of molecular oxygen. Chloroplasts, in contrast, are found only in plants and algae, and are the primary sites of photosynthesis. These organelles work in a different manner to convert energy from the sun into the biosynthesis of required organic nutrients using carbon dioxide and water. Like mitochondria, chloroplasts also contain their own DNA and are able to grow and reproduce independently within the cell. In this digital video, owl monkey kidney epithelial cells (OMK line) were transfected with a mixture of mEGFP fused to a mitochondrial targeting signal (green fluorescence) and DsRed fluorescent protein fused to an endoplasmic reticulum targeting signal (red fluorescence) to observe the interplay between mitochondria and the endoplasmic reticulum.